HiDi® 2x PCR Master Mix

Datasheet

Catalog Number: #9101

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Key Features

Application :

End-Point, Real-Time

SKU Price(USD) Size Qty

#9101M $835.00 500 rxn

Qty:

+ -

#9101S $179.00 100 rxn

Qty:

+ -

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Key Documents

Product Overview

Product Name	HiDi® 2x PCR Master Mix
Catalog Number	#9101
Description	HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination with SYBR chemistry is required. The combination of highly selective aptamer-based fast-start formulated HiDi® DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies. For more information, please check : HiDi® 2x PCR Master Mix
Application	End-Point, Real-Time

Further Specification

Content	S pack: 1 x 1.25 ml HiDi® 2x PCR Master Mix M pack: 5 x 1.25 ml HiDi® 2x PCR Master Mix

Content

S pack: 1 x 1.25 ml HiDi® 2x PCR Master Mix

M pack: 5 x 1.25 ml HiDi® 2x PCR Master Mix

Storage and Shipping

Storage	-20°C
Shipping	Cold packs

References

References	Drum, M., Kranaster, R., Ewald, C., Blasczyk, R., & Marx, A. (2014). Variants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele-and methylation-specific amplification. PloS one, 9(5), e96640. Barraud, N., Létoffé, S., Beloin, C., Vinh, J., Chiappetta, G., & Ghigo, J. M. (2021). Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress. npj Biofilms and Microbiomes, 7(1), 1-11. Miotto, O., Sekihara, M., Tachibana, S. I., Yamauchi, M., Pearson, R. D., Amato, R., ... & Mita, T. (2020). Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea. PLoS pathogens, 16(12), e1009133. Yang, Z., Le, J. T., Hutter, D., Bradley, K. M., Overton, B. R., McLendon, C., & Benner, S. A. (2020). Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems. Biology Methods and Protocols, 5(1), bpaa004. Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., ... & Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nature communications, 10(1), 1-13. Madhuri, S., Bártulos, C. R., Serif, M., Lepetit, B., & Kroth, P. G. (2019). A strategy to complement PtAUREO1a in TALEN knockout strains of Phaeodactylum tricornutum. Algal Research, 39, 101469.

Drum, M., Kranaster, R., Ewald, C., Blasczyk, R., & Marx, A. (2014). Variants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele-and methylation-specific amplification. PloS one, 9(5), e96640.

Barraud, N., Létoffé, S., Beloin, C., Vinh, J., Chiappetta, G., & Ghigo, J. M. (2021). Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress. npj Biofilms and Microbiomes, 7(1), 1-11.

Miotto, O., Sekihara, M., Tachibana, S. I., Yamauchi, M., Pearson, R. D., Amato, R., ... & Mita, T. (2020). Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea. PLoS pathogens, 16(12), e1009133.

Yang, Z., Le, J. T., Hutter, D., Bradley, K. M., Overton, B. R., McLendon, C., & Benner, S. A. (2020). Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems. Biology Methods and Protocols, 5(1), bpaa004.

Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., ... & Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nature communications, 10(1), 1-13.

Madhuri, S., Bártulos, C. R., Serif, M., Lepetit, B., & Kroth, P. G. (2019). A strategy to complement PtAUREO1a in TALEN knockout strains of Phaeodactylum tricornutum. Algal Research, 39, 101469.