HiDi® DNA polymerase

Product Overview

Product Name	HiDi® DNA polymerase
Catalog Number	#9001
Description	HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA). Comparison studies with competitor products show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization. Applications: - Allele-specific PCR (asPCR), allele-specific amplification (ASA) - HLA genotyping - Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP) - Mutation detection by PCR even in a high background of wild-type sequences - Genotyping e.g., in CRISPR/Cas and TALEN approaches This polymerase is also available as a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.). Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below. For research use and further manufacturing. Designed and manufactured under ISO13485
Application	End-Point, Real-Time

Further Specification

Content	S pack: 250 U, 5 U/µl, 1 x 50 µl HiDi® DNA polymerase; 1 x 1.25 ml 10x HiDi reaction buffer M pack: 250 U, 5 U/µl, 1 x 200 µl HiDi® DNA polymerase; 2 x 1.25 ml 10x HiDi reaction buffer

Content

S pack: 250 U, 5 U/µl, 1 x 50 µl HiDi® DNA polymerase; 1 x 1.25 ml 10x HiDi reaction buffer

M pack: 250 U, 5 U/µl, 1 x 200 µl HiDi® DNA polymerase; 2 x 1.25 ml 10x HiDi reaction buffer

Storage and Shipping

Storage	-20°C
Shipping	Cold packs

References

References	Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress. Barraud N, Létoffé S, Beloin C, Vinh J, Chiappetta G, Ghigo JM.NPJ Biofilms Microbiomes. 2021 Apr 13;7(1):34. Link to publication: https://doi.org/10.1038/s41522-021-00203-w Primordial Germ Cell Migration and Histological and Molecular Characterization of Gonadal Differentiation in Pachón Cavefish Astyanax mexicanus Imarazene B, Beille S, Jouanno E, Branthone A, Thermes V, Thomas M, Herpin A, Rétaux S, Guiguen Y Sex Dev. 2021 Mar 10;1-18. Link to publication: https://doi.org/10.1159/000513378 Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea. Miotto O, Sekihara M, Tachibana SI, Yamauchi M, Pearson RD, Amato R, Gonçalves S, Mehra S, Noviyanti R, Marfurt J, Auburn S, Price RN, Mueller I, Ikeda M, Mori T, Hirai M, Tavul L, Hetzel MW, Laman M, Barry AE, Ringwald P, Ohashi J, Hombhanje F, Kwiatkowski DP, Mita T. PLoS Pathog. 2020 Dec 15;16(12):e1009133. Link to publication: https://doi.org/10.1371/journal.ppat.1009133 Rapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing. Yokouchi Y, Suzuki S, Ohtsuki N, Yamamoto K, Noguchi S, Soejima Y, Goto M, Ishioka K, Nakamura I, Suzuki S, Takenoshita S, Era T. Sci Rep. 2020 Aug 18;10(1):13927. Link to publication: https://doi.org/10.1038/s41598-020-70401-7 Development of sake yeast haploid set with diverse brewing properties using sake yeast strain Hiroshima no. 6 exhibiting sexual reproduction. Yamasaki R, Goshima T, Oba K, Kanai M, Ohdoi R, Hirata D, Akao T. J Biosci Bioeng. 2020 Jun;129(6):706-714. Link to publication: https://doi.org/10.1016/j.jbiosc.2020.01.005 Influence of EGFR-activating mutations on sensitivity to tyrosine kinase inhibitors in a KRAS mutant non-small cell lung cancer cell line. Tsukumo Y, Naito M, Suzuki T. PLoS One. 2020 Mar 4;15(3):e0229712. Link to publication: https://doi.org/10.1371/journal.pone.0229712 Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems. Yang Z, Le JT, Hutter D, Bradley KM, Overton BR, McLendon C, Benner SA. Biol Methods Protoc. 2020 Feb 10;5(1):bpaa004. Link to publication: https://doi.org/10.1093/biomethods/bpaa004 Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation. Matsunari H, Watanabe M, Hasegawa K, Uchikura A, Nakano K, Umeyama K, Masaki H, Hamanaka S, Yamaguchi T, Nagaya M, Nishinakamura R, Nakauchi H, Nagashima H. Stem Cell Reports. 2020 Jan 14;14(1):21-33 Link to publication: https://doi.org/10.1016/j.stemcr.2019.11.008 A New Protocol for the Detection of Sterigmatocystin-producing Aspergillus Section Versicolores Using a High Discrimination Polymerase. Kubosaki A, Kobayashi N, Watanabe M, Yoshinari T, Takatori K, Kikuchi Y, Hara-Kudo Y, Terajima J, Sugita-Konishi Y. Biocontrol Sci. 2020 Jan;25(2):113-118 Link to publication: https://doi.org/10.4265/bio.25.113 CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H, Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda J, Mashimo T. Nat Commun. 2019 Dec 6;10(1):5302 Link to publication: https://doi.org/10.1038/s41467-019-13226-x END-phenomenon negative bovine viral diarrhea virus that induces the host's innate immune response supports propagation of BVDVs with different immunological properties. Shiokawa M, Omatsu T, Katayama Y, Nishine K, Fujimoto Y, Uchiyama S, Kameyama KI, Nagai M, Mizutani T, Sakoda Y, Fukusho A, Aoki H. Virology. 2019 Dec;538:97-110 Link to publication: https://doi.org/10.1016/j.virol.2019.09.016 Loss of ALBINO3b Insertase Results in Truncated Light-Harvesting Antenna in Diatoms. Nymark M, Volpe C, Hafskjold MCG, Kirst H, Serif M, Vadstein O, Bones AM, Melis A, Winge P. Plant Physiol. 2019 Nov;181(3):1257-1276 Link to publication: https://doi.org/10.1104/pp.19.00868 Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum. Buck JM, Sherman J, Bártulos CR, Serif M, Halder M, Henkel J, Falciatore A, Lavaud J, Gorbunov MY, Kroth PG, Falkowski PG, Lepetit B. Nat Commun. 2019 Sep 13;10(1):4167 Link to publication: https://doi.org/10.1038/s41467-019-1 2 043-6 Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase. Sakurai T, Kamiyoshi A, Takei N, Watanabe S, Sato M, Shindo T. Sci Rep. 2019 Jul 9;9(1):9923 Link to publication: https://doi.org/10.1038/s41598-019-46357-8 A strategy to complement PtAUREO1a in TALEN knockout strains of Phaeodactylum tricornutum S Madhuri, CR Bártulos, M Serif, B Lepetit, PG Kroth Algal Research, 2019 May, 101469 Link to publication: https://doi.org/10.1016/j.algal.2019.101469 GPR31-dependent dendrite protrusion of intestinal CX3CR1+ cells by bacterial metabolites. Morita N, Umemoto E, Fujita S, Hayashi A, Kikuta J, Kimura I, Haneda T, Imai T, Inoue A, Mimuro H, Maeda Y, Kayama H, Okumura R, Aoki J, Okada N, Kida T, Ishii M, Nabeshima R, Takeda K. Nature. 2019 Feb;566(7742):110-114 Link to publication: https://doi.org/10.1038/s41586-019-0884-1 Precise and efficient nucleotide substitution near genomic nick via noncanonical homology-directed repair. Nakajima K, Zhou Y, Tomita A, Hirade Y, Gurumurthy CB, Nakada S. Genome Res. 2018 Feb;28(2):223-230 Link to publication: https://doi.org/10.1101/gr.226027.117 One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing. Serif M, Dubois G, Finoux AL, Teste MA, Jallet D, Daboussi F. Nat Commun. 2018 Sep 25;9(1):3924 Link to publication: https://doi.org/10.1038/s41467-018-06378-9

Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress.
Barraud N, Létoffé S, Beloin C, Vinh J, Chiappetta G, Ghigo JM.NPJ Biofilms Microbiomes. 2021 Apr 13;7(1):34.
Link to publication: https://doi.org/10.1038/s41522-021-00203-w

Primordial Germ Cell Migration and Histological and Molecular Characterization of Gonadal Differentiation in Pachón Cavefish Astyanax mexicanus
Imarazene B, Beille S, Jouanno E, Branthone A, Thermes V, Thomas M, Herpin A, Rétaux S, Guiguen Y
Sex Dev. 2021 Mar 10;1-18.
Link to publication: https://doi.org/10.1159/000513378

Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea.
Miotto O, Sekihara M, Tachibana SI, Yamauchi M, Pearson RD, Amato R, Gonçalves S, Mehra S, Noviyanti R, Marfurt J, Auburn S, Price RN, Mueller I, Ikeda M, Mori T, Hirai M, Tavul L, Hetzel MW, Laman M, Barry AE, Ringwald P, Ohashi J, Hombhanje F, Kwiatkowski DP, Mita T.
PLoS Pathog. 2020 Dec 15;16(12):e1009133.
Link to publication: https://doi.org/10.1371/journal.ppat.1009133

Rapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing.
Yokouchi Y, Suzuki S, Ohtsuki N, Yamamoto K, Noguchi S, Soejima Y, Goto M, Ishioka K, Nakamura I, Suzuki S, Takenoshita S, Era T.
Sci Rep. 2020 Aug 18;10(1):13927.
Link to publication: https://doi.org/10.1038/s41598-020-70401-7

Development of sake yeast haploid set with diverse brewing properties using sake yeast strain Hiroshima no. 6 exhibiting sexual reproduction.
Yamasaki R, Goshima T, Oba K, Kanai M, Ohdoi R, Hirata D, Akao T.
J Biosci Bioeng. 2020 Jun;129(6):706-714.
Link to publication: https://doi.org/10.1016/j.jbiosc.2020.01.005

Influence of EGFR-activating mutations on sensitivity to tyrosine kinase inhibitors in a KRAS mutant non-small cell lung cancer cell line.
Tsukumo Y, Naito M, Suzuki T.
PLoS One. 2020 Mar 4;15(3):e0229712.
Link to publication: https://doi.org/10.1371/journal.pone.0229712

Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems.
Yang Z, Le JT, Hutter D, Bradley KM, Overton BR, McLendon C, Benner SA.
Biol Methods Protoc. 2020 Feb 10;5(1):bpaa004.
Link to publication: https://doi.org/10.1093/biomethods/bpaa004

Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation.
Matsunari H, Watanabe M, Hasegawa K, Uchikura A, Nakano K, Umeyama K, Masaki H, Hamanaka S, Yamaguchi T, Nagaya M, Nishinakamura R, Nakauchi H, Nagashima H.
Stem Cell Reports. 2020 Jan 14;14(1):21-33
Link to publication: https://doi.org/10.1016/j.stemcr.2019.11.008

A New Protocol for the Detection of Sterigmatocystin-producing Aspergillus Section Versicolores Using a High Discrimination Polymerase.
Kubosaki A, Kobayashi N, Watanabe M, Yoshinari T, Takatori K, Kikuchi Y, Hara-Kudo Y, Terajima J, Sugita-Konishi Y.
Biocontrol Sci. 2020 Jan;25(2):113-118
Link to publication: https://doi.org/10.4265/bio.25.113

CRISPR-Cas3 induces broad and unidirectional genome editing in human cells.
Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H, Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda J, Mashimo T.
Nat Commun. 2019 Dec 6;10(1):5302
Link to publication: https://doi.org/10.1038/s41467-019-13226-x

END-phenomenon negative bovine viral diarrhea virus that induces the host's innate immune response supports propagation of BVDVs with different immunological properties.
Shiokawa M, Omatsu T, Katayama Y, Nishine K, Fujimoto Y, Uchiyama S, Kameyama KI, Nagai M, Mizutani T, Sakoda Y, Fukusho A, Aoki H.
Virology. 2019 Dec;538:97-110
Link to publication: https://doi.org/10.1016/j.virol.2019.09.016

Loss of ALBINO3b Insertase Results in Truncated Light-Harvesting Antenna in Diatoms.
Nymark M, Volpe C, Hafskjold MCG, Kirst H, Serif M, Vadstein O, Bones AM, Melis A, Winge P.
Plant Physiol. 2019 Nov;181(3):1257-1276
Link to publication: https://doi.org/10.1104/pp.19.00868

Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum.
Buck JM, Sherman J, Bártulos CR, Serif M, Halder M, Henkel J, Falciatore A, Lavaud J, Gorbunov MY, Kroth PG, Falkowski PG, Lepetit B.
Nat Commun. 2019 Sep 13;10(1):4167
Link to publication: https://doi.org/10.1038/s41467-019-1 2 043-6

Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase.
Sakurai T, Kamiyoshi A, Takei N, Watanabe S, Sato M, Shindo T.
Sci Rep. 2019 Jul 9;9(1):9923
Link to publication: https://doi.org/10.1038/s41598-019-46357-8

A strategy to complement PtAUREO1a in TALEN knockout strains of Phaeodactylum tricornutum
S Madhuri, CR Bártulos, M Serif, B Lepetit, PG Kroth
Algal Research, 2019 May, 101469
Link to publication: https://doi.org/10.1016/j.algal.2019.101469

GPR31-dependent dendrite protrusion of intestinal CX3CR1+ cells by bacterial metabolites.
Morita N, Umemoto E, Fujita S, Hayashi A, Kikuta J, Kimura I, Haneda T, Imai T, Inoue A, Mimuro H, Maeda Y, Kayama H, Okumura R, Aoki J, Okada N, Kida T, Ishii M, Nabeshima R, Takeda K.
Nature. 2019 Feb;566(7742):110-114
Link to publication: https://doi.org/10.1038/s41586-019-0884-1

Precise and efficient nucleotide substitution near genomic nick via noncanonical homology-directed repair.
Nakajima K, Zhou Y, Tomita A, Hirade Y, Gurumurthy CB, Nakada S.
Genome Res. 2018 Feb;28(2):223-230
Link to publication: https://doi.org/10.1101/gr.226027.117

One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing.
Serif M, Dubois G, Finoux AL, Teste MA, Jallet D, Daboussi F.
Nat Commun. 2018 Sep 25;9(1):3924
Link to publication: https://doi.org/10.1038/s41467-018-06378-9

HiDi® DNA polymerase

Catalog Number: #9001

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