Molecular Reagents

HiFi DNA Polymerase Hot-Start is an aptamer-based hot-start, high fidelity hyperthermophilic recombinant DNA polymerase from the archaeon Pyrococcus furiosus. HiFi DNA Polymerase Hot-Start exhibits 5' to 3' polymerase activity and 3' to 5' exonuclease activity. The optimized buffer chemistry facilitates high sensitivity, yield, specificity, robust and rapid polymerase processivity. The enzyme is ideal for long, complex, difficult DNA templates and is resistant to PCR inhibitors. HiFi DNA Polymerase Hot-Start has a lower error rate than standard Taq DNA polymerase (100x) and therefore, is suitable for PCR applications where higher accuracy is needed.

HighScript Reverse Transcriptase is a modified version of MMLV reverse transcriptase with noticeable thermostability and high enzymatic activity. This enzyme is offered as a blend with an RNase inhibitor to prevent RNA degradation. HighScript Reverse Transcriptase together with enhanced buffer chemistry enables fast, efficient and accurate synthesis of the cDNA molecule.

Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activitiy, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C.

Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

qGreen Mix is a universal intercalating dye mix for robust, sensitive, and fast qPCR. qGreen Mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq polymerase and intercalating dye for real-time PCR amplification. The optimized buffer chemistry and PCR enhancers and stabilizers enable rapid and sensitive qPCR.

qProbe Mix is a universal one-step probe mix for robust, sensitive, and fast qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase for real-time PCR amplification of single or multiplex DNA targets. qProbe Mix is compatible with several probes such as TaqMan® and Scorpions®.

qRT-Probe Mix is a universal one-step probe mix for robust, sensitive, and fast RT-qPCR. The mix uses state-of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase and reverse transcriptase for efficient cDNA synthesis and real-time. The kit includes a ROX additive and an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. qRT-Probe Mix is compatible with several probes such as TaqMan® and Scorpions®.

HiPlex qRT-Probe Mix is an advanced formulated one-step qRT-PCR probe mix for highly sensitive, rapid, and robust detection of RNA target templates. HiPlex qRT-Probe Mix uses state of-the-art technologies with an antibody-regulated hot-start Taq DNA polymerase and ultra-sensitive reverse transcriptase for efficient cDNA synthesis and real-time PCR amplification. HiPlex qRT-Probe Mix is formulated as a 4x mix, which enables extensive multiplexing and addition of larger volumes of RNA templates to reactions. It includes an efficient thermostable reverse transcriptase with RNase inhibitor to prevent degradation of RNA templates by RNases. HiPlex qRT-Probe Mix is compatible with several probes such as TaqMan® and Scorpions®.

Fast Bst Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

Fast Bst RT Mix is a ready-to-use mix containing recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The Bst DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. Fast Bst RT Mix also contains an efficient thermostable reverse transcriptase with an RNase inhibitor (RTase Amp) to prevent degradation of RNA templates by RNases. Fast Bst RT Mix is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.