Molecular Reagents

Taq DNA polymerase Hot-Start is an aptamer-based fast-start formulated DNA polymerase supplied with a 10x reaction buffer that has been specially designed for optimal PCR performance and DNA polymerase activity. It's "Hot-Start" feature eliminates false amplification at temperatures below 50-55°C. 

This DNA polymerase is suitable for a wide range of PCR applications and it's 5'-3'-exonuclease activity means it can be used for hydrolysis probe-based real-time PCRs.

For research use and further manufacturing. Designed and manufactured under ISO13485

Taq 2x PCR Master Mix is a ready-to-use reaction mix for sensitive, robust and fast PCR. The combination of aptamer-based fast-start Taq DNA polymerase, optimized reaction buffer and ulrapure dNTPs ensures consistent results, rapid set-up time and lower risk of pipetting errors. The Taq DNA polymerase has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. It provides a robust PCR performance for a wide range of PCR applications in all standard PCR cyclers.

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Taq 2x PCR Master Mix

qPCR Probe LyoBeads are ready-to-use, freeze-dried master mix beads with all necessary components for rapid and sensitive PCR. The combination of aptamer-based fast-start formulated DNA polymerase, optimized reaction buffer and ultrapure dNTPs in a freeze-dried format results in a cost efficient and ecological master mix that can be rehydrated within seconds in any aqueous solutions. LyoBeads can be shipped and stored at room temperature. Supplied in high profile PCR tubes (0.2 mL) or supplied in low profile PCR tubes (0.1 mL).

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qPCR Probe LyoBeads, pre-dispensed in separable 96x PCR well-plate

DirectBlood Genotyping PCR Kit enables sensitive, rapid and reproductible real-time PCR detection of a wide range of SNPs from EDTA blood samples without prior DNA extraction. 2x DirectBlood Genotyping PCR Mix includes an engineered, aptamer-based fast-start DNA polymerase, optimized reaction buffer and ultrapure dNTPs.

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DirectBlood Genotyping PCR Kit

Volcano3G® RT-PCR Probe 2x Master Mix has all necessary components for sensitive and reliable RT-qPCRs. It includes an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. With the combination of optimized reaction buffer, ultrapure dNTPs and a blue stain for visualization, Volcano3G® RT-PCR Probe 2x Master Mix reduces the need for sample extraction and sample lysis. Volcano3G® RT-PCR Probe 2x Master Mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification.

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Volcano3G® RT-PCR Probe 2x Master Mix

Volcano3G® RT-PCR Probe 2x Master Mix has all necessary components for sensitive and reliable RT-qPCRs. It includes an aptamer-based fast-start formulated enzyme blend of a robust Taq DNA polymerase and an engineered thermostable Volcano3G® DNA polymerase with reverse transcriptase activity. With the combination of optimized reaction buffer, ultrapure dNTPs and a blue stain for visualization, Volcano3G® RT-PCR Probe 2x Master Mix reduces the need for sample extraction and sample lysis. Volcano3G® RT-PCR Probe 2x Master Mix enables amplification of RNA target sequences with quick and easy PCR protocols, even including "zero-step" amplification. Supplied with high ROX concentration (500 nM) or low high ROX concentration (50 nM).

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Volcano3G® RT-PCR Probe 2x Master Mix (+high ROX)

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA).

Comparison studies with competitor products show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. 

By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization.

Applications:

- Allele-specific PCR (asPCR), allele-specific amplification (ASA)

- HLA genotyping

- Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)

- Mutation detection by PCR even in a high background of wild-type sequences

- Genotyping e.g., in CRISPR/Cas and TALEN approaches

This polymerase is also available as a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.).

Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below. 

For research use and further manufacturing. Designed and manufactured under ISO13485

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA).

Comparison studies with competitor products show that the HiDi® Taq DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. 

By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization.

Applications:

- Allele-specific PCR (asPCR), allele-specific amplification (ASA)

- HLA genotyping

- Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)

- Mutation detection by PCR even in a high background of wild-type sequences

- Genotyping e.g., in CRISPR/Cas and TALEN approaches

This DNA polymerase is also available as a nuclease deficient variant, featuring higher robustness towards potential PCR inhibitors and compatibility with real-time dyes.

Several independently conducted studies show that HiDi® Taq DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below. 

For research use and further manufacturing. Designed and manufactured under ISO13485

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination with SYBR chemistry is required. The combination of highly selective aptamer-based fast-start formulated HiDi® DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® 2x PCR Master Mix

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination is required. The combination of highly selective aptamer-based fast-start formulated HiDi® Taq DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. The variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.

For more information, please check :
HiDi® Taq 2x PCR Master Mix