Molecular Reagents

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA).

Comparison studies with competitor products show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. 

By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization.

Applications:

- Allele-specific PCR (asPCR), allele-specific amplification (ASA)

- HLA genotyping

- Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)

- Mutation detection by PCR even in a high background of wild-type sequences

- Genotyping e.g., in CRISPR/Cas and TALEN approaches

This polymerase is also available as a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.).

Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below. 

For research use and further manufacturing. Designed and manufactured under ISO13485

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This enzyme family is optimized for this feature and is the first choice for applications that rely on this property such as allele-specific PCR (asPCR), also known as allele-specific amplification (ASA).

Comparison studies with competitor products show that the HiDi® Taq DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. 

By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10,000 wild-type copies without any other tedious assay optimization.

Applications:

- Allele-specific PCR (asPCR), allele-specific amplification (ASA)

- HLA genotyping

- Analysis of single CpG methylation sites by PCR (methylation specific PCR, MSP)

- Mutation detection by PCR even in a high background of wild-type sequences

- Genotyping e.g., in CRISPR/Cas and TALEN approaches

This DNA polymerase is also available as a nuclease deficient variant, featuring higher robustness towards potential PCR inhibitors and compatibility with real-time dyes.

Several independently conducted studies show that HiDi® Taq DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. Please see "References" below. 

For research use and further manufacturing. Designed and manufactured under ISO13485

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination with SYBR chemistry is required. The combination of highly selective aptamer-based fast-start formulated HiDi® DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

For more information, please check :
HiDi® 2x PCR Master Mix

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq 2x PCR Master Mix is a ready-to-use master mix specially optimized for assays in which high discrimination is required. The combination of highly selective aptamer-based fast-start formulated HiDi® Taq DNA polymerase and an optimized buffer with ultrapure dNTPs ensures simple reaction setup and relible results. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. The variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® Taq DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10^4 wild-type copies.

For more information, please check :
HiDi® Taq 2x PCR Master Mix

5 µM, 20 µl
For more information, please check :
2'-O-Me sensitive RT-KTQ DNA polymerase mutant

5 µM, 20 µl
For more information, please check :
RT-KTQ2 DNA polymerase

5 µM, 20 µl
For more information, please check :
m6A sensitive RT-KTQ DNA polymerase mutant

All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!

• Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
• Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
• Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood. 
• Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.
• Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried.

For research use and further manufacturing. Designed and manufactured under ISO13485

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable, aptamer-based fast-formulated reverse transcriptase and combined DNA polymerase with a half-life at 95°C of > 40 min. This enzyme allows reverse transcription reactions at high temperatures, minimizing the problems encountered with strong secondary structures in RNA. RevTaq RT-PCR DNA polymerase allows “zero-step” RT-PCRs directly from RNA templates without an isothermal reverse transcription step, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes.

For more information, please check :
RevTaq RT-PCR DNA polymerase, 50x concentrated glycerol stock

qPCR Probe 2x Lyocake Master Mix is a ready-to-use reaction mix for sensitive and reliable probe-based qPCR in all standard real-time PCR cyclers. It includes an engineered, aptamer-based fast-start DNA polymerase, optimized reaction buffer and ultrapure dNTPs. Freeze-dried qPCR Probe 2x Lyocake Master Mix can be stored at room temperature.

For more information, please check :
qPCR Probe 2 x LyoCake Master Mix (freeze-dried)