RevTaq RT-PCR DNA polymerase
Catalog Number: #6500
End-Point, Real-Time
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Key Documents
Product Name | RevTaq RT-PCR DNA polymerase |
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Catalog Number | #6500 |
Description | RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable, aptamer-based fast-formulated reverse transcriptase and combined DNA polymerase with a half-life at 95°C of > 40 min. This enzyme allows reverse transcription reactions at high temperatures, minimizing the problems encountered with strong secondary structures in RNA. RevTaq RT-PCR DNA polymerase allows “zero-step” RT-PCRs directly from RNA templates without an isothermal reverse transcription step, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes. For more information, please check : |
Application | End-Point, Real-Time |
Content | S pack: 1 x 50 µl RevTaq RT-PCR DNA polymerase (50x); 1 x 1.25 ml 5x Volcano buffer L pack: 1 x 250 µ RevTaq RT-PCR DNA polymerase (50x); 2 x 1.25 ml 5x Volcano buffer |
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Storage | -20°C |
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Shipping | Cold packs |
References | Blatter, N., Bergen, K., Nolte, O., Welte, W., Diederichs, K., Mayer, J., ... & Marx, A. (2013). Structure and Function of an RNA‐Reading Thermostable DNA Polymerase. Angewandte Chemie International Edition, 52(45), 11935-11939. |
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