RevTaq RT-PCR DNA polymerase

Catalog Number: #6500

Key Features

End-Point, Real-Time

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Key Documents

Product Overview
Product Name RevTaq RT-PCR DNA polymerase
Catalog Number #6500
Description

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable, aptamer-based fast-formulated reverse transcriptase and combined DNA polymerase with a half-life at 95°C of > 40 min. This enzyme allows reverse transcription reactions at high temperatures, minimizing the problems encountered with strong secondary structures in RNA. RevTaq RT-PCR DNA polymerase allows “zero-step” RT-PCRs directly from RNA templates without an isothermal reverse transcription step, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes.

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RevTaq RT-PCR DNA polymerase, 50x concentrated glycerol stock

Application End-Point, Real-Time
Further Specification
Content

S pack: 1 x 50 µl RevTaq RT-PCR DNA polymerase (50x); 1 x 1.25 ml 5x Volcano buffer

L pack: 1 x 250 µ RevTaq RT-PCR DNA polymerase (50x); 2 x 1.25 ml 5x Volcano buffer

Storage and Shipping
Storage -20°C
Shipping Cold packs
References
References Blatter, N., Bergen, K., Nolte, O., Welte, W., Diederichs, K., Mayer, J., ... & Marx, A. (2013). Structure and Function of an RNA‐Reading Thermostable DNA Polymerase. Angewandte Chemie International Edition, 52(45), 11935-11939.